The match was originally scheduled for Murrayfield but after representations from Glasgow supporters the third leg of the series will be played at the Warriors' home which will have an increased capacity.
The first two matches will remain as a double-header over the Christmas and New Year period, and will be played at Murrayfield and Scotstoun respectively.
Originally a two-legged event, with each side playing home and away, the format was altered to three matches following the introduction of South African teams to the PRO14.
Glasgow Warriors managing director Nathan Bombrys said: "I know the Warrior Nation will be looking forward to hosting Edinburgh at Scotstoun on the final day of the Guinness PRO14 season.
"I am confident, knowing the passion of our fans, it will be a fantastic occasion with a real cup final atmosphere and we will be doing all we can to win back the 1872 Cup from our close Scottish rivals and secure a league match victory in the process."
Last year, 'Silver Saturday' played host to the 1872 Cup final and the event will retain the traditional Cup Finals day format for the coming season with fans attending able to watch the Glasgow Warriors vs Edinburgh Rugby match on big screens at Murrayfield.