Australian international Nick Frisby makes his first appearance in a Warriors jersey - the 25-year-old starts at scrum-half alongside Brandon Thomson.
Lelia Masaga starts on one wing with former Harlequin Robbie Nairn named on the other. Scotland international Rory Hughes completes the back three wearing number 15.
Patrick Kelly and Sam Johnson complete the backline, combining in midfield.
Scotland International Jonny Gray starts in the engine room alongside Scott Cummings. New Academy graduate Bruce Flockhart joins Chris Fusaro and Matt Smith in the back-row.
Up front, Oli Kebble and D'arcy Rae start either side of James Malcolm.
There are seven internationals among the replacements including USA's Greg Peterson and Fiji's Nikola Matawalu.
Alex Allan, Robert Harley, Adam Hastings, Alex Dunbar and Ruaridh Jackson are the Scotland internationals named on the bench.
Currie Chieftans' Joe Reynolds is also included having spent time training with the Warriors during pre-season.
Glasgow Warriors Head Coach Dave Rennie said: "Our selection reflects us trying to give the guys who have been training since day one of pre-season an opportunity to showcase their ability against a good Quins side.
"It's a squad full of good young men who have worked really hard and we're looking forward to seeing them under a bit of heat.
"We've had a couple of international guys who didn't tour in since the beginning and we were keen to give Jonny a run before round one. More guys will come in next week against Northampton.
"It'll be exciting for the supporters to see Nick Frisby too, he's a good man who has fitted in really well. It's a good opportunity for him to show everyone what he can do in front of what promises to be a really good crowd in Perth."
Glasgow Warriors team to play Harlequins in the Famous Grouse Pre-Season Challenge at North Inch, Perth - Saturday 18 August, 2.00pm.
1 Oli Kebble
2 James Malcolm
3 D'arcy Rae
4 Scott Cummings
5 Jonny Gray
6 Bruce Flockhart
7 Chris Fusaro
8 Matt Smith
9 Nick Frisby
10 Brandon Thomson
11 Lelia Masaga
12 Patrick Kelly
13 Sam Johnson
14 Robbie Nairn
15 Rory Hughes
Replacements
Kevin Bryce, Grant Stewart, Alex Allan, Adam Nicol, Greg Peterson, Robert Harley, Tom Gordon, Kaleem Barreto, Adam Hastings, Ratu Tagive, Stafford McDowall, Alex Dunbar, Nikola Matawalu, Joe Reynolds and Ruaridh Jackson