Robert Harley and George Turner have both been released by Scotland this weekend. The hooker is flanked by Oli Kebble and D'arcy Rae in the front-row, while Scott Cummings is Harley's partner in the engine room.
On the bench Kiran McDonald returns from injury to be involved for the first time his season.
Glasgow Warriors Head Coach Dave Rennie said: "We're excited by the challenge this week. What we know is that regardless of who pulls on the jersey there is an expectation to perform in it and we've named a strong squad that can go out there and deliver.
"Ospreys are right on our tails in the Conference and are playing really well. We'll need to reach the same levels of physicality we have done in our last three games to get the performance we expect from a Glasgow Warriors side.
"It's tough for Sam (Johnson), he's just returned from injury so to injure his other knee is disappointing for him. He's a determined man and has already begun work on his rehab and we look forward to having him back fit."
16 Grant Stewart
17 Jamie Bhatti
18 Adam Nicol
19 Kiran McDonald
20 Matt Smith
21 Bruce Flockhart
22 Patrick Kelly
23 Nikola Matawalu
Unavailable players: Zander Fagerson (ankle), Siua Halanukonuka (foot), Stuart Hogg (ankle), Sam Johnson (knee), Lelia Masaga (back) and Tim Swinson (lower limb).
Alex Allan (Scotland), Fraser Brown (Scotland), Alex Dunbar (Scotland), Matt Fagerson (Scotland), Jonny Gray (Scotland), Adam Hastings (Scotland), George Horne (Scotland), Peter Horne (Scotland), Huw Jones (Scotland), Lee Jones (Scotland), Greg Peterson (USA), Ali Price (Scotland), Tommy Seymour (Scotland) and Ryan Wilson (Scotland)