The 28-year-old signed for Glasgow in 2010 and has been a key member of the squad ever since, scoring a try in the triumphant 2015 PRO12 Final.
Just as comfortable playing in the back-row as he is in the second-row, Harley broke Graeme Morrison's club appearance record against Munster last season and will reach another milestone when he leads the team out on Sunday.
Wilson and Harley both joined the Warriors at the same time and Harley made his debut coming on for Wilson against Leinster at Firhill.
Glasgow Warriors Head Coach Dave Rennie said: "Rob and Ryan are both bloody good men who are really passionate about this club.
"They're good quality people and are highly respected within the group. It'll be a special day for them and their families.
"Obviously the last three weeks have been disappointing, but we've created a lot of opportunities in those games and just haven't been as clinical as we've needed to be and the quality of our decision making hasn't been good enough.
"It's hurt us but there's no sense of panic, we need to be sharper and we know this is a massive game for us in the context of Europe and we've picked the side accordingly."
15 Stuart Hogg
Players unavailable through injury: Fraser Brown (knee), Alex Dunbar (thigh), Zander Fagerson (ankle), Jonny Gray (shoulder), Siua Halanukonuka (calf), Peter Horne (knee), Nikola Matawalu (illness), Robbie Nairn (knee) and George Turner (ankle)