There are nine changes in total to the side that claimed a 20-7 victory over Scarlets last time out, with Wilson still able to select a matchday 23 containing 16 internationals for the visit to Swansea.
An all-new starting front row sees Aki Seiuli and D'arcy Rae named as the starting props, packing down either side of Scotland hooker George Turner.
Hamish Bain - set for his Glasgow debut - is joined in the second-row by Richie Gray, who became the latest player to reach 50 appearances for the club on his last outing in Glasgow colours.
Ryan Wilson captains the side from the blindside flank, as Tom Gordon continuing at openside in a back-row completed another debutant - Samoan international TJ Ioane.
Jamie Dobie is handed his first start of the 2020/21 campaign at scrum-half, with the 2019/20 Young Player of the Season partnered with Peter Horne in the half-backs.
There's a return to action in the midfield for Sam Johnson after his recent injury layoff, the 27-year-old joining Nick Grigg in the centres.
An unchanged back three sees Ratu Tagive and Tommy Seymour start on the wings, with Huw Jones retaining the 15 jersey.
Sean Kennedy is in line to make his first competitive appearance for Glasgow Warriors in over seven years should he come off the bench tomorrow evening, the scrum-half's previous outing for the club having come in February 2013 during a short-term loan spell.
There could also be first appearances of the season for Alex Allan, Grant Stewart and Brandon Thomson, the latter having scored all of Glasgow's points in their most recent meeting with the Ospreys at Scotstoun in 2019.
Argentina prop Enrique Pieretto and Tongan back-rower Fotu Lokotui are both included in the 23 for the second away fixture in succession, having both been included in the squad that took on Connacht in Galway three weeks ago.
The bench is completed by Nikola Matawalu, who has fond memories of his most recent trip to the Liberty Stadium; the Fijian crossed for a hat-trick in November 2018 en route to helping his team claim a bonus-point victory.
Speaking to glasgowwarriors.org, Head Coach Danny Wilson said: "I was pleased with our showing in first hour of our win against Scarlets. It was an improvement on our performance away to Connacht.
"The team includes some new combinations that we are looking forward to seeing in action.
"It's good to welcome back a couple of the boys who have been released to us from Scotland camp.
"TJ [Ioane] and Hamish [Bain] will be making their debut for the club, and it will be the first time I get to see Sam [Johnson] and Jamie [Dobie] in a Warriors shirt.
"Ospreys have started off the season well. They have a good mix of young talent and experienced veterans in their squad - similar to us.
"We've prepared well and are up for the challenge we'll face tomorrow in Swansea."
15 Huw Jones
14 Tommy Seymour
13 Nick Grigg
12 Sam Johnson
11 Ratu Tagive
10 Peter Horne
9 Jamie Dobie
1 Aki Seiuli
2 George Turner
3 D'arcy Rae
4 Hamish Bain
5 Richie Gray
6 Ryan Wilson (Captain)
7 Tom Gordon
8 TJ Ioane
Substitutes
16 Grant Stewart
17 Alex Allan
18 Enrique Pieretto
19 Fotu Lokotui
20 Chris Fusaro
21 Sean Kennedy
22 Brandon Thomson
23 Nikola Matawalu