The Glasgow contingent includes five players hoping to make their debuts in Scotland colours, including Rufus McLean; the winger currently leads the United Rugby Championship in clean breaks with six after four rounds.
Sione Tuipulotu is also included amongst Townsend's midfield options, having crossed for his first try for the Warriors in last weekend's victory over Zebre Parma as the latest part of an eye-catching start to his Glasgow career.
Half-back duo Jamie Dobie and Ross Thompson retain their spaces after linking up with the squad for a two-day training camp earlier this week, the former having also been a part of the Scotland squad for the conclusion of the Guinness Six Nations earlier in 2021.
The uncapped quintet is completed by Rory Darge, whose early season form sees him in contention for an international bow in the number seven shirt.
Elsewhere, Danny Wilson's squad provides six of the front-row options available to Townsend and his coaching team, with Fraser Brown and George Turner both included at hooker.
Jamie Bhatti and Oli Kebble are selected after both men featured in the away win in Parma, whilst Murray McCallum will look to add to his tally of Scotland caps from 2018 after joining Zander Fagerson amongst the front-row ranks.
Robert Harley's experience sees the club's record holder for appearances included in the engine room, with Matt Fagerson looking to build on his Guinness Six Nations form for Scotland at number eight.
The Warriors provide all three of the scrum-halves in the 42-man playing group, as Ali Price and George Horne join Dobie in the stable of number nines chosen by Townsend.
Sam Johnson returns to the fold after returning to action at the start of the season in impressive style, whilst Kyle Steyn completes the Glasgow contingent as he looks to add to his single Scotland cap to date from the win over France at BT Murrayfield in March 2020.
Scotland open their campaign against Tonga at BT Murrayfield next weekend, before facing Australia, South Africa and Japan on successive weekends at the home of Scottish rugby.
Autumn Nations Series Squad
Forwards:
Ewan Ashman - Sale Sharks
Josh Bayliss - Bath Rugby
Jamie Bhatti - Glasgow Warriors
Fraser Brown - Glasgow Warriors
Luke Crosbie - Edinburgh Rugby
Rory Darge - Glasgow Warriors
Matt Fagerson - Glasgow Warriors
Zander Fagerson - Glasgow Warriors
Grant Gilchrist - Edinburgh Rugby
Nick Haining - Edinburgh Rugby
Robert Harley - Glasgow Warriors
Jamie Hodgson - Edinburgh Rugby
Oli Kebble - Glasgow Warriors
Murray McCallum - Glasgow Warriors
Stuart McInally - Edinburgh Rugby
Dylan Richardson - Cell C Sharks
Jamie Ritchie - Edinburgh Rugby
Sam Skinner - Exeter Chiefs
Pierre Schoeman - Edinburgh Rugby
Rory Sutherland - Worcester Warriors
Marshall Sykes - Edinburgh Rugbyl
George Turner - Glasgow Warriors
Hamish Watson - Edinburgh Rugby
Backs:
Mark Bennett - Edinburgh Rugby
Darcy Graham - Edinburgh Rugby
Jamie Dobie - Glasgow Warriors
Chris Harris - Gloucester Rugby
Adam Hastings - Gloucester Rugby
Stuart Hogg - Exeter Chiefs
George Horne - Glasgow Warriors
Damien Hoyland - Edinburgh Rugby
Sam Johnson - Glasgow Warriors
Huw Jones - Harlequins
Blair Kinghorn - Edinburgh Rugby
Duhan van de Merwe - Worcester Warriors
Rufus McLean - Glasgow Warriors
Ali Price - Glasgow Warriors
Finn Russell - Racing 92
Matt Scott - Leicester Tigers
Kyle Steyn - Glasgow Warriors
Ross Thompson - Glasgow Warriors
Sione Tuipulotu - Glasgow Warriors