Smith - who scored on his Murrayfield debut against Australia in the 2022 Autumn Nations Series - is one of 10 Warriors selected in Gregor Townsend's matchday 23.
Sione Tuipulotu is one of seven Warriors to have featured in each of Scotland's five matches in the championship, with the centre also handed the vice-captaincy by Head Coach Townsend after consistently impressing in the competition thus far.
Huw Jones once again lines up outside his club-mate, as he looks to add to his championship-leading tally of four tries to date.
Kyle Steyn retains his place on the wing after an industrious showing throughout the championship, the Glasgow skipper having also started in last year's clash with the Azzurri at the Stadio Olimpico.
Up front, Jack Dempsey continues at number eight after impressing on his first Guinness Six Nations start last weekend.
Zander Fagerson makes his fourth consecutive start since returning from injury, with the tight-head the most experienced Warrior in the starting XV as he prepares to win his 58th cap.
George Turner packs down alongside Fagerson in the front-row, having started each match in the 2023 competition.
Three more Warriors line up on the bench, including Scott Cummings after a 75-minute performance against Ireland on his first Scotland appearance since returning from injury.
Ali Price retains the 21 jersey, the scrum-half having sparked the free-flowing move that led to Chris Harris's World Rugby Try of the Year nominee in Rome last season.
Matt Fagerson completes the Glasgow contingent as he rotates to the bench for the first time in the championship, the back-rower's tally of 70 tackles enough to see him sit atop the standings after four rounds of action.
Scotland host the first match of this year's Super Saturday, with kick-off at Murrayfield scheduled for 12.30pm.
Scotland
15 Ollie Smith (Glasgow Warriors) 2 caps
14 Kyle Steyn (Glasgow Warriors) 9 caps
13 Huw Jones (Glasgow Warriors) 35 caps
12 Sione Tuipulotu (Glasgow Warriors) - Vice Captain - 15 caps
11 Duhan van der Merwe (Edinburgh Rugby) 27 caps
10 Blair Kinghorn (Edinburgh Rugby) 42 caps
9 Ben White (London Irish) 13 caps
1 Pierre Schoeman (Edinburgh Rugby) 20 caps
2 George Turner (Glasgow Warriors) 34 caps
3 Zander Fagerson (Glasgow Warriors) 57 caps
4 Sam Skinner (Edinburgh Rugby) 24 caps
5 Jonny Gray (Exeter Chiefs) - Vice-Captain - 76 caps
6 Jamie Ritchie (Edinburgh Rugby) - Captain - 40 caps
7 Hamish Watson (Edinburgh Rugby) 56 caps
8 Jack Dempsey (Glasgow Warriors) 8 caps
Substitutes
16 Ewan Ashman (Sale Sharks) 6 caps
17 Rory Sutherland (Ulster Rugby) 22 caps
18 WP Nel (Edinburgh Rugby) 53 caps
19 Scott Cummings (Glasgow Warriors) 24 caps
20 Matt Fagerson (Glasgow Warriors) 32 caps
21 Ali Price (Glasgow Warriors) 60 caps
22 Ben Healy (Munster) uncapped
23 Cam Redpath (Bath) 4 caps