Tuipulotu and Jones are set to start together in the British & Irish Lions' midfield for the first time.
Jones retains his starting spot at outside centre after starting against the Queensland Reds on Wednesday while Tuipulotu is at 12 after being rested for the midweek win.
Scott Cummings also returns to the matchday 23 as one of six forwards on Andy Farrell's bench meaning for the second time on tour all three Warriors tourists have been named in the same squad.
Former Glasgow prop Tom Lambert lines up for the Waratahs with Mahe Vailanu - younger brother of Sione - among the replacements.
British & Irish Lions
15 Hugo Keenan (Leinster Rugby/ Ireland)
14 Mack Hansen (Connacht Rugby/ Ireland)
13 Huw Jones (Glasgow Warriors/ Scotland)
12 Sione Tuipulotu (Glasgow Warriors/ Scotland)
11 Blair Kinghorn (Toulouse/ Scotland)
10 Fin Smith (Northampton Saints/ England)
9 Alex Mitchell (Northampton Saints/ England)
1 Pierre Schoeman (Edinburgh Rugby/ Scotland)
2 Luke Cowan-Dickie (Sale Sharks/ England)
3 Finlay Bealham (Connacht Rugby/ Ireland)
4 Tadhg Beirne (Munster Rugby/ Ireland)(Captain)
5 James Ryan (Leinster Rugby/ Ireland)
6 Henry Pollock (Northampton Saints/ England)
7 Josh van der Flier (Leinster Rugby/ Ireland)
8 Ben Earl (Saracens/ England)
Substitutes
16 Dan Sheehan (Leinster Rugby/ Ireland)
17 Ellis Genge (Bristol Bears/ England)
18 Tadhg Furlong (Leinster Rugby/ Ireland)
19 Joe McCarthy (Leinster Rugby/ Ireland)
20 Scott Cummings (Glasgow Warriors/ Scotland)
21 Jac Morgan (Ospreys/ Wales)
22 Ben White (Toulon/ Scotland)
23 Marcus Smith (Harlequins/ England)