Warriors took an early lead through Briar McNamara's try in their first home game this campaign but were 33 points behind just after the second-half restart after Wolfhounds showed their experience and quality.
Ceitidh Ainsworth and Emily Coubrough scored second-half tries as Warriors but Wolfhounds were to good and san out 64-21 winners.
The result leaves Glasgow second bottom of the competition table, level on points with bottom side Brython Thunder.
15 Sky Phimister
14 Abi Evans
13 Briar McNamara
12 Carla McDonald
11 Roma Fraser
10 Ceitidh Ainsworth
9 Mairi McDonald
1 Ailie Tucker (Captain)
2 Aicha Sutcliffe
3 Katie Lindsay
4 Holland Bogan
5 Aisha Zameer
6 Freya Walker
7 Eilidh MacGilvray
8 Emily Coubrough
Substitutes
16 Megan Hyland
17 Poppy Clarkson
18 Louise Downie
19 Kate Yeomans
20 Sophie Price
21 Mary Nelson
22 Poppy Mellanby
23 Orla Proctor