The match with Ulster is expected to sell out and will be played in front of around 10,000 supporters at Scotstoun Stadium (ko7.45pm) – live on Sky Sports 3 & BBC ALBA.
Glasgow Warriors finished top of the GUINNESS PRO12 for the first time in the club's history and are unbeaten at home in the league since November 2013.
This game will be the final home match for skipper Alastair Kellock, Nikola Matawalu and DTH van der Merwe, with the Canadian international named among the replacements and set to return from injury.
Coming into the side which started the 32-10 win over Ulster last Saturday is scrum-half Henry Pyrgos and after being named McCrea Financial Services Player of the Season last night at the club's Awards Dinner, Niko Matawalu moves from scrum-half to the left wing in place of Sean Lamont.
In the forwards Fraser Brown returns to the line-up after recovering from a hamstring injury and he replaces Dougie Hall at hooker.
A limited number of tickets and hospitality places are available for tomorrow's game from glasgowwarriors.org
Gregor Townsend said: "It will be a big challenge facing one of the best sides in Europe for the second time in a week and it's knock-out rugby now so we know what's at stake.
"We've had some epic battles with Ulster over the last few seasons and they'll be eager to reverse the scoreline from last week.
"Tomorrow we expect to be playing in front of another sell-out crowd at Scotstoun for a place in another final and everyone at the club is really looking forward to the match."
15 Stuart Hogg
14 Tommy Seymour
13 Richie Vernon
12 Peter Horne
11 Nikola Matawalu
10 Finn Russell
9 Henry Pyrgos
1 Ryan Grant
2 Fraser Brown
3 Rossouw de Klerk
4 Jonny Gray
6 Alastair Kellock (Captain)
6 Josh Strauss
7 Chris Fusaro
8 Adam Ashe
Substitutes
16 Pat MacArthur
17 Gordon Reid
18 Michael Cusack
19 Leone Nakarawa
20 Ryan Wilson
21 Duncan Weir
22 Sean Lamont
23 DTH van der Merwe
Not available due to injury: Alex Allan (shoulder), Mark Bennett (shoulder), Hugh Blake (ankle), Alex Dunbar (knee), Tyrone Holmes (knee), Sean Maitland (shoulder) and Tim Swinson (ankle)