Glasgow prop Sila Puafisi will face his former club and is looking forward to returning to Kingsholm with the PRO12 side.
Sila told glasgowwarriors.org: "It's always a pleasure to see my friends and former team-mates from Gloucester, and it's going to be good to play against them.
"I enjoyed my time there. I made some close friends and I'm excited to go back. I know they're looking forward to playing against us!
"It'll be a really tough game. Kingsholm is always a tough place to go to and Gloucester are a very good team.
"They have really good supporters, especially in the famous Shed, so it'll be a good atmosphere."
15 Rory Hughes
14 Leonardo Sarto
13 Alex Dunbar
12 Sam Johnson
11 Sean Lamont
10 Peter Horne
9 Henry Pyrgos (Captain)
1 Gordon Reid
2 Corey Flynn
3 Zander Fagerson
4 Greg Peterson
5 Scott Cummings
6 Robert Harley
7 Fraser Brown
8 Ryan Wilson
Replacements: Ryan Grant, Pat MacArthur, D'arcy Rae, Sila Puafisi, Tim Swinson, Tjiuee Uanivi, Simone Favaro, Lewis Wynne, Ali Price, Grayson Hart, Rory Clegg, Richie Vernon, Fraser Lyle