Stafford McDowall is the only uncapped Warrior selected by Townsend in the 41-strong playing group, the centre rewarded after impressing in both the URC and Challenge Cup.
Sione Tuipulotu and Huw Jones join McDowall amongst the midfield options, the pair having been selected in the official Guinness Six Nations Team of the Tournament after their performances earlier this year.
Club captain Kyle Steyn is named as one of five back-three players chosen by Townsend after featuring in all five of Scotland's Six Nations clashes in 2023, with Ollie Smith joining Steyn following his first Six Nations start in the win over Italy in March.
A trio of Glasgow Warriors scrum-halves are selected by Townsend, as Jamie Dobie - whose single cap to date came in a 60-14 win over Tonga at BT Murrayfield back in October 2021 - joins Ali Price and George Horne amongst the options in the number nine shirt.
Up front, Rory Darge's eye-catching form since his return from injury sees the openside selected as one of seven back-rowers named by the coaching group, with club-mates Matt Fagerson and Jack Dempsey joining Darge in the back-row cohort.
Having reached a century of Glasgow Warriors appearances against Munster last weekend, Richie Gray is named in the second-row alongside club-mate, and fellow recent centurion, Scott Cummings.
George Turner is the sole Glasgow hooker selected by Townsend, with fellow Guinness Six Nations regulars Zander Fagerson and Jamie Bhatti chosen amongst the props.
Murphy Walker completes the Warriors contingent, the Dundee-born front-rower looking to add to his two Scotland caps to date.
The 41-player group will gather for an initial training camp on 29 May to begin preparations for warm-up matches in the Famous Grouse Nations Series against France, Italy and Georgia at BT Murrayfield in July and August. Scotland also play France in an away warm-up in St. Etienne in August.
The Rugby World Cup itself will see Scotland compete in Pool B alongside South Africa, Ireland, Tonga and Romania, with a first fixture against the world champion Springboks on Sunday 10 September in Marseille.
Scotland training squad for Rugby World Cup 2023
Forwards (23)
Ewan Ashman (Sale Sharks) 7 caps
Jamie Bhatti (Glasgow Warriors) 28 caps
Dave Cherry (Edinburgh Rugby) 8 caps
Andy Christie (Saracens) 4 caps
Luke Crosbie (Edinburgh Rugby) 4 caps
Scott Cummings (Glasgow Warriors) 25 caps
Rory Darge (Glasgow Warriors) 7 caps
Jack Dempsey (Glasgow Warriors) 9 caps
Matt Fagerson (Glasgow Warriors) 33 caps
Zander Fagerson (Glasgow Warriors) 58 caps
Grant Gilchrist (Edinburgh Rugby) 62 caps
Richie Gray (Glasgow Warriors) 73 caps
Cameron Henderson (Leicester Tigers) uncapped
Stuart McInally (Edinburgh Rugby) 47 caps
WP Nel (Edinburgh Rugby) 54 caps
Jamie Ritchie (Edinburgh Rugby) - CAPTAIN - 41 caps
Pierre Schoeman (Edinburgh Rugby) 21 caps
Javan Sebastian (Scarlets) 3 caps
Sam Skinner (Edinburgh Rugby) 25 caps
Rory Sutherland (Ulster Rugby) 23 caps
George Turner (Glasgow Warriors) 35 caps
Murphy Walker (Glasgow Warriors) 2 caps
Hamish Watson (Edinburgh Rugby) 57 caps
Backs (18)
Jamie Dobie (Glasgow Warriors) 1 cap
Darcy Graham (Edinburgh Rugby) 33 caps
Chris Harris (Gloucester Rugby) 42 caps
Adam Hastings (Gloucester Rugby) 27 caps
Ben Healy (Munster Rugby) 1 caps
Stuart Hogg (Exeter Chiefs) 100 caps
George Horne (Glasgow Warriors) 20 caps
Huw Jones (Glasgow Warriors) 36 caps
Blair Kinghorn (Edinburgh Rugby) 43 caps
Stafford McDowall (Glasgow Warriors) uncapped
Ali Price (Glasgow Warriors) 61 caps
Cameron Redpath (Bath Rugby) 5 caps
Finn Russell (Racing 92) 69 caps
Ollie Smith (Glasgow Warriors) 3 caps
Kyle Steyn (Glasgow Warriors) 10 caps
Sione Tuipulotu (Glasgow Warriors) 16 caps
Duhan van der Merwe (Edinburgh Rugby) 28 caps
Ben White (London Irish) 14 caps