Glasgow Warriors duo Rory Sutherland and Gregor Brown, along with Darcy Graham and Ewan Ashman, turned out for the Lions in their 24-19 win on Tuesday.
Graham went over for a try before being forced off with an injury after 16 minutes.
Brown replaced James Ryan for the last 25 minutes and showed up well while Ashman and Sutherland came on for the final six minutes to help close out a hard-fought win.
With no further injuries to the squad in their positions, it has been confirmed the trio have now left the squad alongside the injured Graham.