Duncan Munn is included in the Boroughmuir Bears squad on a partnership contract with Glasgow Warriors.
The Scotland U20 centre was part of Glasgow's academy group over the last two seasons.
Kaleen Barreto is also included in the Bears' squad.
Scrum-half Ben Afshar was part of Glasgow's academy group last season and will join Stirling Wolves on a partnership deal. Afshar spent last season with Ayrshire Bulls and is currently skippering Scotland U20 in the World Rugby Trophy in Kenya.
Winger Ross McKnight is back with Stirling after spending last season with Watsonians.
Three Warriors are also included in the squad as transition players - Max Williamson, Logan Trotter and Gregor Brown.
Jamie Drummond and Andy Stirrat were part of Glasgow's academy group last season and are both included in the Ayrshire Bulls Championship squad.
Glasgow's Alex Samuel, Nathan McBeth and Ross Thompson are listed as transition players.
Fraser Brown will combine his Glasgow duties with a coaching role at Watsonians for the Super Series Championship.