A top four spot is guaranteed meaning they will be at home for the play-off quarter-finals but the likelihood is that they will be on the road if they wish to progress any further in the competition.
Munster, Bulls and Leinster sit above Glasgow in the URC standings but only three points separating the top four. Glasgow take on bottom club Zebre at Scotstoun in the final round, while Leinster host Connacht, the Bulls travel to Durban to play the Sharks and Munster take on Ulster in Limerick.
After the excitement of topping the standings the 44-21 defeat to 14-man Lions in Johannesburg was a blow to players, staff and fans but club captain Kyle Steyn says the back-to-back defeats in South Africa would not compromise the progress that went before.
"It's not demoralising, it is just disappointing," said Steyn. "There had been a massive amount of work behind the scenes done to make sure we were prepared for these two games from the coaches and staff.
"There were lots of lessons for us to take on the chin and look at," said Steyn.
"It will be a fairly honest review. There will be no hints of complacency. [Zebre] is the last regular season game at home in front of our home fans so there is plenty to play for."
Potentially playing a semi-final and final away is challenging but Steyn cited the example of last season's champions Munster who played all three of their play-off games on the road.
"We would have loved to have played as many games [as we can] at Scotstoun and to play those games in front of our fans but you look at last year Munster won all their three games away from home so it can be done," said Steyn. "It is a test of character for this group now and we can't look too far ahead to that semi-final because we have a game at home against Zebre and then the quarter-finals so there is lots to focus on until we get there."