New signing Jarrod Firth started for Stirling County against Ayr at Bridgehaugh. Great work from Sam Johnson and Nick Grigg got County off to a good start but Ayr came back to take the 32-18 win.
County weren't helped by the sending off of hooker Cameron Fenton.
Back from injury, Javan Sebastian burrowed over for a try and D'arcy Rae came off the bench for the visitors.
Glasgow Hawlks also advance after a 64-5 win over city rivals GHA. The game saw a return to action for highly-rated Academy Stage 3 forward Kiran McDonald after a long injury lay-off.