Sione Tuipulotu will resume his captaincy of the side, having led the men in blue during last November's Test matches before missing the Guinness Six Nations due to injury and touring with the British & Irish Lions this summer.
The captain is joined by four more of Glasgow's Lions amongst the playing group, as Scott Cummings and Zander Fagerson join Gregor Brown and Rory Sutherland - the latter two having featured against the First Nations & Pasifika XV as late call-ups to the tour - amongst the contingent of forwards selected.
Murphy Walker is selected in a Scotland squad for the first time since the 2024 summer tour of the Americas, with Nathan McBeth also selected in the squad after returning from injury in Saturday's victory over the Ospreys.
The starting Glasgow second-row from that win in Bridgend is also selected, as Alex Samuel - who made his senior international debut against Portugal in last November's international window - and Max Williamson are included in Townsend's playing group.
Rory Darge and Jack Dempsey are both included as they continue their return from their respective injuries, whilst Matt Fagerson - who made 20 tackles in Bridgend on Saturday night - completes the cohort of Glasgow forwards selected for Scotland.
Behind the scrum, Jamie Dobie is selected alongside George Horne as two of Townsend's options at scrum-half, the former having crossed for two tries in a BKT URC Player of the Match-winning display.
Adam Hastings will look to add to his 34 caps for Scotland to date, whilst Stafford McDowall - who captained Scotland against the Maori All Blacks this summer - joins Tuipulotu amongst the options at centre.
Ollie Smith is included after an impressive start to the 2025/26 campaign that has seen him run out at full-back, centre and wing so far this season, with Kyle Rowe and Kyle Steyn rounding out the Warriors competing for Test minutes this autumn.
Scotland squad
Backs: Fergus Burke (Saracens), Jamie Dobie (Glasgow Warriors) Darcy Graham (Edinburgh), Adam Hastings (Glasgow Warriors), Rory Hutchinson (Northampton Saints), George Horne (Glasgow Warriors), Tom Jordan (Bristol Bears), Blair Kinghorn (Toulouse), Stafford McDowall (Glasgow Warriors), Kyle Rowe (Glasgow Warriors), Arron Reed (Sale Sharks), Finn Russell (Bath), Ollie Smith (Glasgow Warriors), Kyle Steyn (Glasgow Warriors), Ross Thompson (Edinburgh), Sione Tuipulotu (Glasgow Warriors), Duhan van der Merwe (Edinburgh), Ben White (Toulon).
Forwards: Ewan Ashman (Edinburgh), Josh Bayliss (Bath), Magnus Bradbury (Edinburgh), Gregor Brown (Glasgow Warriors), Scott Cummings (Glasgow Warriors), Rory Darge (Glasgow Warriors), Jack Dempsey (Glasgow Warriors), Matt Fagerson (Glasgow Warriors), Zander Fagerson (Glasgow Warriors), Grant Gilchrist (Edinburgh), Patrick Harrison (Edinburgh), Nathan McBeth (Glasgow Warriors), Alexander Masibaka (Montpellier), Liam McConnell (Edinburgh Rugby), Elliot Millar Mills (Northampton Saints), Harri Morris (Edinburgh), Andy Onyeama-Christie (Saracens), D'Arcy Rae (Edinburgh), Dylan Richardson (Edinburgh), Jamie Ritchie (Perpignan), Alex Samuel (Glasgow Warriors), Pierre Schoeman (Edinburgh Rugby), Rory Sutherland (Glasgow Warriors), Marshall Sykes (Edinburgh), George Turner (Harlequins), Murphy Walker (Glasgow Warriors), Max Williamson (Glasgow Warriors)